Advances in Biochemical Engineering, Volume 9 by V. L. Yarovenko (auth.)

By V. L. Yarovenko (auth.)

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Tropicalis were substantially desorbed from the hydrocarbon phase at a centrifugal force greater than 10000 x g, while about one fourth of the adsorbed cells of C. intermedia were desorbed due to a centrifugal force greater than 3 000 × g. The effect of the surface active agent, Tween 20, on the adhesive force between the cell and hydrocarbon is shown in Fig. 1. Figure 1 also shows that all adsorbed Fig. 1. Degree of Desorption of cells from n-tetradecane under centrifugal forces [81 ~ I/ ~" 0 1 ~ 0 o--- ~x~ I , 5 I0 CEliTRIFUGAL FORCE ZlO-a(g) 15 o C.

Aiba et aL [9] proposed the following Monod-type model for the microbial 44 Y. /max KS + S* where S* designates the concentration of accommodated oil. 6 If the value of S* is in a fairly small range compared with Ks, the specific growth rate, as expressed using Eqs. 24 ~' ) - 0"6 (3) where fl = proportionality constant. e. the number of cells per unit volume of dispersion which can be attached to droplets, and Kp is the saturation constant for the potential. 5 + d*) d*. (5) When the diameter of the oil droplets is much smaller than that of the cells, the specific growth rate can be derived from Eqs.

C~ I--Gt. Fig. 12. Oxygen consumption by n-tetradecane-grown C. ~ c~ 0 0 I TIME { h r } The rate of oxygen uptake by hydrocarbon-precultured cells on drop-form hydrocarbon was approximately equal to that of alanine-precultured cells. For C. intermedia, which had a strong adhesive force with hydrocarbon, the maximum rate of oxygen uptake on accommodation-form hydrocarbon was nearly equal to that on drop-form hydrocarbon: Qo~ (AH)max - Qo, (DH)max For C. tropicalis, which had a low adhesive force to hydrocarbon, the maximum rate of oxygen uptake on accommodation-form hydrocarbon was higher than that on drop-form hydrocarbon: Qo 2 (AH)max > Qo~ (DH)max.

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